Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114006_yk57 AATTATTCAATCACCTGGAAAAAAAAGTCTTG(+CAGTGT)GTACCAATAAAATATGTTTTTCTGTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240605114004_w14z GAAAGAAGATGACAGAAAAACATATTTTATTG(+CAGTGT)GTACCAAGACTTTTTTTTCCAGGTGATTGAA NGG 10 8 17 8 24 0 100 5 10
    240605114003_w1cf TAAAATCAAATGAAGGTTTAAGATCACATAAG(+CAGTGT)GATCCTGCTTCTGTTAGTTAAGGAAACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605114002_kk9l CTTAGAGCAGTTTCCTTAACTAACAGAAGCAG(+CAGTGT)GATCCTTATGTGATCTTAAACCTTCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605114000_9lgo TGATGCAGCTGACTGATGAAGGTACTGGTGAG(+CAGTGT)GTACCTCTAGGGCTAGGACCAGATGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605113959_74ft TGAAGTCACTGCATCTGGTCCTAGCCCTAGAG(+CAGTGT)GTACCTCACCAGTACCTTCATCAGTCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605113957_454j GTCTTCTTCTCTGCCAGATAACAGAGCCATTG(+CAGTGT)GATCCACTTATTAAAGACTTCTTATCACTTA NGG 10 8 17 8 24 0 100 5 10
    240605113956_ycp8 AATGTTAAGTGATAAGAAGTCTTTAATAAGTG(+CAGTGT)GATCCAATGGCTCTGTTATCTGGCAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605113954_qtbd CTTTTTCTAGAGTCCTTTCTACACACACTTTG(+CAGTGT)GTACCCTGGGGAGTTTGGATGCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605113951_vfts CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+CAGTGT)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10