Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113013_l3cl TGGATTCGTATGGCTAACGTCAGTGAATCAAG(+CAGTGT)GATCCCAGGGGATGCCCATTGCTGGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240605113010_f1qp AACTGAGATGCCCAGCAATGGGCATCCCCTGG(+CAGTGT)GATCCTTGATTCACTGACGTTAGCCATACGA NGG 10 8 17 8 24 0 100 5 10
    240605113008_5c2i ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+CAGTGT)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605113007_ca32 GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+CAGTGT)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605113005_8v1j TCTACATTTTAATTTTTATGATTCCTGATGTG(+CAGTGT)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605113004_tw6v ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+CAGTGT)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605113002_qnix GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+CAGTGT)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605113000_sik0 CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+CAGTGT)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605112958_lrkp AAAAATTACTAAAACCATGCAAAATGATTGAG(+CAGTGT)GATCCCAAAGGCTTTCTTAATGTACACTAGT NGG 10 8 17 8 24 0 100 5 10
    240605112957_9cv5 TAGATACTAGTGTACATTAAGAAAGCCTTTGG(+CAGTGT)GATCCTCAATCATTTTGCATGGTTTTAGTAA NGG 10 8 17 8 24 0 100 5 10