Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112924_r969 GCCTCTGTGTAGTCTGGATCTACTGACACTGG(+CAGTGT)GTACCAGGGTTTCCTTCTGTTGACTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240605112921_m5hj GCTGCAATCACAGTCAACAGAAGGAAACCCTG(+CAGTGT)GTACCCAGTGTCAGTAGATCCAGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240605112920_nynb GTGGAATTTCTCCAGGGTGGAATTTTAGACTG(+CAGTGT)GTACCAGAAGTCGGCAAGGCGTGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605112919_9x2u TGTGTCCAGCAGTCACGCCTTGCCGACTTCTG(+CAGTGT)GTACCAGTCTAAAATTCCACCCTGGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605112918_6f70 CTACCACTAAGCTATACTTTCGAACTTTTTAG(+CAGTGT)GATCCTTTGTGCTGGAGGAGAGCAAAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605112917_o5rh AAGGCCCTGCTTTGCTCTCCTCCAGCACAAAG(+CAGTGT)GATCCTAAAAAGTTCGAAAGTATAGCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605112915_b5yt ATCCAGGTTCTATGGCCAGCTGGCCACGAAGG(+CAGTGT)GTACCTTGGTCCTAGGCAACCCACCTCTCGG NGG 10 8 17 8 24 0 100 5 10
    240605112913_p20m GAGAGCCGAGAGGTGGGTTGCCTAGGACCAAG(+CAGTGT)GTACCCTTCGTGGCCAGCTGGCCATAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605112911_qdkq CATATCAAGGACAGGGTGAGGAAGTGGCCGTG(+CAGTGT)GATCCAGCTGAGAGTGGCAGCCTCGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605112910_q838 GCATCCAGTCCCGAGGCTGCCACTCTCAGCTG(+CAGTGT)GATCCACGGCCACTTCCTCACCCTGTCCTTG NGG 10 8 17 8 24 0 100 5 10