Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112853_ud51 CTTCAGTGAAGCATGCCAGAGGCAGTGAACGG(+CAGTGT)GTACCATAAGTGGCTGCTGGGATATACCAGG NGG 10 8 17 8 24 0 100 5 10
    240605112852_u5su GCCTACCTGGTATATCCCAGCAGCCACTTATG(+CAGTGT)GTACCCGTTCACTGCCTCTGGCATGCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605112849_hybn GAGGGTTTTCAGTTGTGACAGCCCCACCCCCG(+CAGTGT)GTACCATCTCTCTCCTCAATGTAGTCATGCA NGG 10 8 17 8 24 0 100 5 10
    240605112848_d35d GTGCTTGCATGACTACATTGAGGAGAGAGATG(+CAGTGT)GTACCGGGGGTGGGGCTGTCACAACTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605112846_t8l3 CTGGGGCCAATAAGCTGGCTTAGTAGGTAAAG(+CAGTGT)GTACCTGCTGCCAAGCCTGATGCCCTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605112845_kyya GATTGAACTCAGGGCATCAGGCTTGGCAGCAG(+CAGTGT)GTACCTTTACCTACTAAGCCAGCTTATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605112844_aqx5 TTGCCTGCGTTTATGTCTGTGTGAGAGTTTTG(+CAGTGT)GATCCCCTAAAACGAGTTGCAAGCAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605112842_3o4b TAACTCACACCTGCTTGCAACTCGTTTTAGGG(+CAGTGT)GATCCAAAACTCTCACACAGACATAAACGCA NGG 10 8 17 8 24 0 100 5 10
    240605112840_29dj AATAAGCCAGGCATGGTGCCTGGGGGCAGGAG(+CAGTGT)GATCCTGACTAGAAAGCGATTGGTCTGCATA NGG 10 8 17 8 24 0 100 5 10
    240605112839_9fit CTCACTATGCAGACCAATCGCTTTCTAGTCAG(+CAGTGT)GATCCTCCTGCCCCCAGGCACCATGCCTGGC NGG 10 8 17 8 24 0 100 5 10