Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112751_2ozl TCCCTGCTCAAATTATGCCTCTTAGGATAAAG(+CAGTGT)GTACCCAGCATTTTGTTTTGGGTGTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605112749_8r98 ACTGTATTTTCACACCCAAAACAAAATGCTGG(+CAGTGT)GTACCTTTATCCTAAGAGGCATAATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605112747_f3s8 AACACCTTTGAAAAAAAAAAAAAAAGCCTACG(+CAGTGT)GTACCACTTTTATACATAGAACCCACTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605112745_uzcj CCTGGGTTAAGTGGGTTCTATGTATAAAAGTG(+CAGTGT)GTACCGTAGGCTTTTTTTTTTTTTTTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605112744_73jz CCATTGTTCCAAGGCTGCACATGGCTTGGAAG(+CAGTGT)GATCCTGTTTGAGTGTATGCTTCCTCACTCA NGG 10 8 17 8 24 0 100 5 10
    240605112743_6i9u GGAGCTGAGTGAGGAAGCATACACTCAAACAG(+CAGTGT)GATCCTTCCAAGCCATGTGCAGCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605112741_66wq ACTAGGAACAACTAGTTGAGCAAGTCTTTAAG(+CAGTGT)GTACCGATTGTCAAAATACATCACTAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605112740_klrk TAATTTGTCTTAGTGATGTATTTTGACAATCG(+CAGTGT)GTACCTTAAAGACTTGCTCAACTAGTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605112739_d7dd AATGCCTTCTTCAGTTTACCAGCAGTGGTGTG(+CAGTGT)GTACCAGTGAGGTATTTCAGGTAACGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605112738_kbiq AAGAGAGAGACGTTACCTGAAATACCTCACTG(+CAGTGT)GTACCACACCACTGCTGGTAAACTGAAGAAG NGG 10 8 17 8 24 0 100 5 10