Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605112820_g5mj GTTACAGCAGGGATTTGACAAAACACAGGGTG(+CAGTGT)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605112819_6thh GATCGTTCAAAGTTAAGTTTCCAAACTGCATG(+CAGTGT)GATCCACCCTGTGTTTTGTCAAATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605112817_7x4k TTTCTAAGCCACACAAGATGGGGTAAAAAGGG(+CAGTGT)GTACCAACACCTCAATGGCAGTTAGACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605112816_cepk TGAGTGGAAGTCTAACTGCCATTGAGGTGTTG(+CAGTGT)GTACCCCTTTTTACCCCATCTTGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605112814_fydq ATGTGAATCCAGAGCTTGCTGGGGAGCCAGAG(+CAGTGT)GTACCATTCAATGGCAGAGCTCTTCCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605112813_9ojo CTTATGCTAGGGAAGAGCTCTGCCATTGAATG(+CAGTGT)GTACCTCTGGCTCCCCAGCAAGCTCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605112811_qbvz TCCGATGTCTGATGTGTCATCTTTTTATCTTG(+CAGTGT)GATCCTTCCCTGGTTTGAAACTTTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240605112810_b57a TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+CAGTGT)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10
    240605112808_l64o CCTGTCCTTTAATTCTCAGAACCCCTTTCGAG(+CAGTGT)GTACCTACATTGACTGGTAGTCGAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240605112807_bbsp TGTAGTCGACCCTCGACTACCAGTCAATGTAG(+CAGTGT)GTACCTCGAAAGGGGTTCTGAGAATTAAAGG NGG 10 8 17 8 24 0 100 5 10