Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113950_hbo6 CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+CAGTGT)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605113948_kwre CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+CAGTGT)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605113947_ahsw AAATGGTCCATATAAATTTGTGGCATCAATGG(+CAGTGT)GATCCCAACTTGGCTTCCCTAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605113945_1hup CGTGGGAAGGAAGACTAGGGAAGCCAAGTTGG(+CAGTGT)GATCCCATTGATGCCACAAATTTATATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605113943_e2zi AGGACCATGACAAAGCCCCTGAGATCAAGGTG(+CAGTGT)GTACCAGTTGCAGCAGCAATGTGGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605113942_if0e TAAGGCTGAGCACCACATTGCTGCTGCAACTG(+CAGTGT)GTACCACCTTGATCTCAGGGGCTTTGTCATG NGG 10 8 17 8 24 0 100 5 10
    240605113940_y4c7 TGATGTGACTAGAAAGGCCTGGCCACTTTAGG(+CAGTGT)GTACCAAGGGAGAACTCCAAGGAGCATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605113937_u2xe GGGGGAAAAATGCTCCTTGGAGTTCTCCCTTG(+CAGTGT)GTACCCTAAAGTGGCCAGGCCTTTCTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605113935_swb2 ATTGCCTTATGTAGTAATCAGCAACTGAAGGG(+CAGTGT)GTACCCAACCTCTACAGTCTTCCAGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240605113933_zhvb GGGCAATTGTGCTGGAAGACTGTAGAGGTTGG(+CAGTGT)GTACCCCTTCAGTTGCTGATTACTACATAAG NGG 10 8 17 8 24 0 100 5 10