Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113113_sic2 AATGCTTATGAATATTTGTGATCTATAGGTTG(+CAGTGT)GATCCATAGCAACACCAACACACAAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605113112_lsk2 GATCCCATTTTTTGTGTGTTGGTGTTGCTATG(+CAGTGT)GATCCAACCTATAGATCACAAATATTCATAA NGG 10 8 17 8 24 0 100 5 10
    240605113110_v86i AAAAAGAAGCTTACAATAATGACCTAAGTATG(+CAGTGT)GATCCAGTGGGGTATTCCACCACACAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605113109_tmgv ACCTTTGCACTGTGTGGTGGAATACCCCACTG(+CAGTGT)GATCCATACTTAGGTCATTATTGTAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605113107_65di TGTCTTCCATGCCTCCTGGTGAATCTTGCTTG(+CAGTGT)GTACCATTCAATCCCATGTGTAAAGACAGAC NGG 10 8 17 8 24 0 100 5 10
    240605113106_j4ro GCCCTGTCTGTCTTTACACATGGGATTGAATG(+CAGTGT)GTACCAAGCAAGATTCACCAGGAGGCATGGA NGG 10 8 17 8 24 0 100 5 10
    240605113105_6sl9 GTATATATGTCTGTGCACAGTGCACATGTCTG(+CAGTGT)GTACCTGCATTAACCAGACAGAAGACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605113104_9gqr AGGAGATGGAGTCTTCTGTCTGGTTAATGCAG(+CAGTGT)GTACCAGACATGTGCACTGTGCACAGACATA NGG 10 8 17 8 24 0 100 5 10
    240605113103_78lc TGAAACACAGTTACCATTTTGTGTGGATTTAG(+CAGTGT)GTACCCCAAAATGTTTTTTTAAGTGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605113100_6igr CCTATTTGTTTCACTTAAAAAAACATTTTGGG(+CAGTGT)GTACCTAAATCCACACAAAATGGTAACTGTG NGG 10 8 17 8 24 0 100 5 10