Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113323_aiyg GTGTCTCTTGGCACTGTCACCAAGTTGCCCAG(+CAGTGT)GATCCTGGGGATGCTGTAATTTGGAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605113320_jsvr GCCCTCAATTTTCCAAATTACAGCATCCCCAG(+CAGTGT)GATCCTGGGCAACTTGGTGACAGTGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605113318_okzs CAGCAGCCACTGGGAAGAAGAGGCACAACAAG(+CAGTGT)GATCCATCCTCCCAGAGACCTTTCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240605113317_fs1r TGGCAGGAGTTGGAAAGGTCTCTGGGAGGATG(+CAGTGT)GATCCTTGTTGTGCCTCTTCTTCCCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605113316_t572 TCCTGGGCTTGGGCAGTGAGAGGAGCATCTAG(+CAGTGT)GTACCTGTTGTGCCCCTAATAAAGTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605113314_zt8i TGTCATCCAACACTTTATTAGGGGCACAACAG(+CAGTGT)GTACCTAGATGCTCCTCTCACTGCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605113313_nfyd AATGAGTGCCTTCGCAGCCTGGTAGAAGAGAG(+CAGTGT)GATCCGATTCGTCACCATCAGAATCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240605113312_f0ly TGGGGAAAGTGGATTCTGATGGTGACGAATCG(+CAGTGT)GATCCTCTCTTCTACCAGGCTGCGAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605113311_o2et TGGTTCTAGCCCTTGACAGGTTGCTCCGCGAG(+CAGTGT)GATCCCTGTAGCTCACACACTGGGCCTATGG NGG 10 8 17 8 24 0 100 5 10
    240605113309_afpj GCTGGCCATAGGCCCAGTGTGTGAGCTACAGG(+CAGTGT)GATCCTCGCGGAGCAACCTGTCAAGGGCTAG NGG 10 8 17 8 24 0 100 5 10