Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113351_4pcv GGGGACAGGGGGCACCTGGGGAAGTCCGCAAG(+CAGTGT)GATCCGGGGCTTGGTGAGAGGTGGGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605113349_zgmc ACCCGCAGCTCCTAGTCTCTAATGCCTGGTAG(+CAGTGT)GATCCCATGGGCCTTTGCGACGCCACGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605113347_tccv GTTGCCTAACGTGGCGTCGCAAAGGCCCATGG(+CAGTGT)GATCCTACCAGGCATTAGAGACTAGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605113346_c49w GGACATCAATCTAAAGCCTCGAGGCCATGTTG(+CAGTGT)GATCCACCCTGACCCGGTCCTGTAGCAACTA NGG 10 8 17 8 24 0 100 5 10
    240605113344_huwc TTTTTTAGTTGCTACAGGACCGGGTCAGGGTG(+CAGTGT)GATCCAACATGGCCTCGAGGCTTTAGATTGA NGG 10 8 17 8 24 0 100 5 10
    240605113342_naoq TTTAGCAAAAGAAATAATGTCTTAGTACTTGG(+CAGTGT)GTACCACGGCGAGAGACAGGTCACCTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605113340_n2h6 GCAGGGTGGCAGGTGACCTGTCTCTCGCCGTG(+CAGTGT)GTACCCAAGTACTAAGACATTATTTCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605113339_5iva CTCCTCAGAGGGAGATGACAGGAGTGGAGATG(+CAGTGT)GTACCGATAGTCGTTGCTAGTCTGTCTACCG NGG 10 8 17 8 24 0 100 5 10
    240605113338_d282 ACGCACGGTAGACAGACTAGCAACGACTATCG(+CAGTGT)GTACCATCTCCACTCCTGTCATCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605113336_641z TCGTGGACATGGCAAAACCAGGCAAGAGCCAG(+CAGTGT)GATCCACAGAGAAACCCTCTTGCTTGCTTCT NGG 10 8 17 8 24 0 100 5 10