Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113437_6s6c TCCATCCATTCTCTGGGGCTTCTGGAGAACAG(+CAGTGT)GATCCGATGAAGAAGCAGTGGCCAGAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605113436_kqhn TGAACACCCAGGCCTTTGGGGATGAGCTGCTG(+CAGTGT)GTACCCACTTCTGAAGAGACAGGTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240605113434_4zn1 GCTCCAGTGGCCACCTGTCTCTTCAGAAGTGG(+CAGTGT)GTACCAGCAGCTCATCCCCAAAGGCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605113432_y7eg ACAGGTGGCCACTGGAGCCCTCTTGGTCCCAG(+CAGTGT)GATCCCCCCCTCACCACTCCAGACTTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605113429_5dt9 GTTCAAAGCTAAGTCTGGAGTGGTGAGGGGGG(+CAGTGT)GATCCTGGGACCAAGAGGGCTCCAGTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605113428_wm9e TGTCTAAGACCCATGCTGTGCCAGCCGGCCTG(+CAGTGT)GTACCTATGGGAGTTTTCACGTTCCGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605113426_qd2y ATACACACACCGGAACGTGAAAACTCCCATAG(+CAGTGT)GTACCAGGCCGGCTGGCACAGCATGGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605113424_c0hy ATCGGGGTAAGAGGGCCATTAAGGAGGCCGAG(+CAGTGT)GTACCCTTTCTATGTTCCAAGCAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605113423_54fh CCCACCTCTCTCCTGCTTGGAACATAGAAAGG(+CAGTGT)GTACCTCGGCCTCCTTAATGGCCCTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240605113421_685k AGAAGTCCCTGACAAGATGGTGTTTGGCTTGG(+CAGTGT)GATCCAGAGATGTCCAAATCCTGTCTTCCAC NGG 10 8 17 8 24 0 100 5 10