Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113528_m10v TATTTTTGTGCAAGTGCCCCCCATGGTTGGGG(+CAGTGT)GATCCTGACAGAAATGGGGCAAACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605113526_fzmt GCCCTTAGCATGACGGGTTCTGCCCTTTGTGG(+CAGTGT)GATCCGGTAAGGAAACTGAGAGGTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605113524_8qcw TTGAAACAAGGAACCTCTCAGTTTCCTTACCG(+CAGTGT)GATCCCACAAAGGGCAGAACCCGTCATGCTA NGG 10 8 17 8 24 0 100 5 10
    240605113523_8dh1 ATTATAGGACAGAAAGTGCTGGGGTTGGGGAG(+CAGTGT)GTACCAAGAGGGTGAGTAGTAGGGGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605113522_9rri GGGGAGTCTTCCCCCTACTACTCACCCTCTTG(+CAGTGT)GTACCTCCCCAACCCCAGCACTTTCTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605113520_29j0 ATTGTTCAGTTCTGTGTGTGAGGGTAGGCAGG(+CAGTGT)GTACCTGCCCCATGTCAGCCGCCCTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605113518_g4tj TTCTTCTCATCAGGGCGGCTGACATGGGGCAG(+CAGTGT)GTACCCTGCCTACCCTCACACACAGAACTGA NGG 10 8 17 8 24 0 100 5 10
    240605113517_l6ir TTACCCCAATTGTCATAAGGCCTGAGAGAATG(+CAGTGT)GTACCCACTTTCCCTGTCCCAAAGTGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605113516_va63 CTTTTTGAGGCACTTTGGGACAGGGAAAGTGG(+CAGTGT)GTACCATTCTCTCAGGCCTTATGACAATTGG NGG 10 8 17 8 24 0 100 5 10
    240605113513_wb64 GTCACTGTCCCAAACAGCATAGTAAGCCCATG(+CAGTGT)GTACCAGCTTCCCCAGGGGATGCGGGACACA NGG 10 8 17 8 24 0 100 5 10