Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113547_9lxk GCCTTCAGCGGGGGTGAAGAGCGCAGTGGAAG(+CAGTGT)GTACCCAGGAGGAGAAGAGGGCAAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605113545_xor9 TCCTTGCATGTTTATGCTCTTATGTATTCCTG(+CAGTGT)GTACCCAGGGAGGCCAGAAGAGGGCTGATTC NGG 10 8 17 8 24 0 100 5 10
    240605113542_whem CCAGGGAATCAGCCCTCTTCTGGCCTCCCTGG(+CAGTGT)GTACCAGGAATACATAAGAGCATAAACATGC NGG 10 8 17 8 24 0 100 5 10
    240605113541_0gx0 TGCTGCCGTGTATGTGCTGGGAATTGAACCAG(+CAGTGT)GATCCTCTGGATTTGCAGCTAGAACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605113539_zfha CATGGTTAAAAGTTCTAGCTGCAAATCCAGAG(+CAGTGT)GATCCTGGTTCAATTCCCAGCACATACACGG NGG 10 8 17 8 24 0 100 5 10
    240605113537_08v0 CAGCTTACTGTTGCAGCTGCCTGAGCAGCCTG(+CAGTGT)GATCCTGCCTGAGGTTAGACCAGAGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240605113535_h73e GAGGAGGAGGTCTCTGGTCTAACCTCAGGCAG(+CAGTGT)GATCCAGGCTGCTCAGGCAGCTGCAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240605113534_sfk8 ACCCTAAAGTTTCTAGAAGGAGGCAGGGAAGG(+CAGTGT)GTACCCCAGGCTCCCCATCCCCAGGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605113531_mz1a GGACTCAGCTCCCTGGGGATGGGGAGCCTGGG(+CAGTGT)GTACCCTTCCCTGCCTCCTTCTAGAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605113530_dt70 AAACAGCTTCATGTTTGCCCCATTTCTGTCAG(+CAGTGT)GATCCCCCAACCATGGGGGGCACTTGCACAA NGG 10 8 17 8 24 0 100 5 10