Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110624_e2pu TTACCGGCTGCAGAAGTCAGAAGAGGGCATTG(+CAGTGT)GATCCCTCTGGAACCTAAGTTACAGATGATT NGG 10 8 17 8 24 0 100 5 10
    240605110623_mvd5 AATGTCTGAACCCTGGCTTTCAATTTTTTTAG(+CAGTGT)GTACCCTACTTAGAGATGGACTTGCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605110621_6us0 CATGTAATCCAGCAAGTCCATCTCTAAGTAGG(+CAGTGT)GTACCTAAAAAAATTGAAAGCCAGGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605110619_vqyh ATCTGACTGAGATTTGCCAAGTGGATACTGGG(+CAGTGT)GTACCACTGCCCCCCAAGAAAAGACTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605110618_2mjh TTCCTGGCTCAGTCTTTTCTTGGGGGGCAGTG(+CAGTGT)GTACCCCAGTATCCACTTGGCAAATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605110616_elkn CACAGGGCCCAGATGGTGGGTAACTGAGGCAG(+CAGTGT)GATCCAGACTTGAGACACACGGTTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605110614_3gmi AGCCTTGTTTCCAACCGTGTGTCTCAAGTCTG(+CAGTGT)GATCCTGCCTCAGTTACCCACCATCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605110613_xjta GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+CAGTGT)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605110612_d0y6 GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+CAGTGT)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605110610_8xcd TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+CAGTGT)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10