Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110607_9eor CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+CAGTGT)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240605110605_28oo GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+CAGTGT)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240605110603_mmxg CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+CAGTGT)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605110601_tf4e CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+CAGTGT)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605110600_tnlr ATCAGATTGACACCCAAAACATCCTGTTTTGG(+CAGTGT)GATCCTGAGGCTCCACGATCAAGGTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240605110558_0csc TGGAAGAGTTGTGTCCTTCCCCTACGAAGGTG(+CAGTGT)GTACCGATGTGCTCTGCCTTTCTGATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605110557_0s53 ATAAAATGGGATCAGAAAGGCAGAGCACATCG(+CAGTGT)GTACCACCTTCGTAGGGGAAGGACACAACTC NGG 10 8 17 8 24 0 100 5 10
    240605110555_udfr CACACACGTGCATGCTCTGTGCGTGTATGTGG(+CAGTGT)GTACCGTGTATATGTGTGTGAGTGTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240605110554_6z7a ATGCATATACAACACTCACACACATATACACG(+CAGTGT)GTACCCACATACACGCACAGAGCATGCACGT NGG 10 8 17 8 24 0 100 5 10
    240605110551_dmlp CTACTGATACTTTCAGGGGTGGTCCCATGGGG(+CAGTGT)GATCCATGCAGTGGAAGAAACACTGGATTGG NGG 10 8 17 8 24 0 100 5 10