Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110503_r1mc CCTGCACTGAAAGGATTGAGTTCAGGAGGCAG(+CAGTGT)GATCCATACCACCATCTGACGCCCTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605110501_x0z2 AGTCATTGTTAGGTGTTCGTGTTGGGAATGGG(+CAGTGT)GATCCTTCAAGGAACAGAGTAAAGCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605110500_m5k9 AGGGTGAAGGTGCTTTACTCTGTTCCTTGAAG(+CAGTGT)GATCCCCATTCCCAACACGAACACCTAACAA NGG 10 8 17 8 24 0 100 5 10
    240605110458_y73c TACACCGGGTCTCTTTAAAATTGGCATGCTCG(+CAGTGT)GTACCAAATAGGTGAGCTCATAATTAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605110457_j9c2 CTGTAATTTCTAATTATGAGCTCACCTATTTG(+CAGTGT)GTACCGAGCATGCCAATTTTAAAGAGACCCG NGG 10 8 17 8 24 0 100 5 10
    240605110455_gxtz ATCCAGAGCAGTGATGATGAGCAGGTAGACGG(+CAGTGT)GTACCAGCCGGCGGGCTTCTGGATAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605110454_c6hb ACAGGGACCCCTATCCAGAAGCCCGCCGGCTG(+CAGTGT)GTACCCGTCTACCTGCTCATCATCACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605110452_91bm CTAGCATGAGACTTAGAAGCTCCTGATCCAAG(+CAGTGT)GTACCAGATGTCATAGTTACCTAAGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605110450_lazg AAATTCCTTCTCTTAGGTAACTATGACATCTG(+CAGTGT)GTACCTTGGATCAGGAGCTTCTAAGTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605110449_9u02 TCCCAAACTTAGGGCTTCCTCCATGTGCAAGG(+CAGTGT)GTACCGTCACTGAGACACATCGCTAGCACAA NGG 10 8 17 8 24 0 100 5 10