Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110411_o3kf AGAAAAATAGATTTACCTTACTGTTTCTAGGG(+CAGTGT)GATCCTTTTGTTTGTTTTTGCTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605110410_jjp8 TTAGGTAAAATTATAGGAAAAATTATTGAAAG(+CAGTGT)GTACCCTTCAATGGACAGTGTCATCTATTTC NGG 10 8 17 8 24 0 100 5 10
    240605110408_u1or TACTAGAAATAGATGACACTGTCCATTGAAGG(+CAGTGT)GTACCTTTCAATAATTTTTCCTATAATTTTA NGG 10 8 17 8 24 0 100 5 10
    240605110407_6u0e TCTAGAAAGCATATTTAAAATCGTTGGTATCG(+CAGTGT)GTACCAGGAGAAGCCAGGCTAAGAAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605110404_0dua CAGGACTTTATTTCTTAGCCTGGCTTCTCCTG(+CAGTGT)GTACCGATACCAACGATTTTAAATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605110403_a5hm CACAGCCATGCCCAGCTTTTTGTGAGTGCTGG(+CAGTGT)GATCCAAACTCAGGTACTTCTTACCCACTGA NGG 10 8 17 8 24 0 100 5 10
    240605110401_2cu6 ATGTCTCAGTGGGTAAGAAGTACCTGAGTTTG(+CAGTGT)GATCCCAGCACTCACAAAAAGCTGGGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605110400_d721 ACTGTCTTTACACACACTAGAAGAGGGCATTG(+CAGTGT)GATCCCATTACATGTGGTTGCTGGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240605110358_mazq TGAGTTCAATTCCCAGCAACCACATGTAATGG(+CAGTGT)GATCCAATGCCCTCTTCTAGTGTGTGTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605110357_u9nl TGACCCGTGCTTTGAGAACTCGGGAAAACCAG(+CAGTGT)GATCCATGTCCACTTCACTTCGACTTTATCT NGG 10 8 17 8 24 0 100 5 10