Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110447_975f TAAGCTTGTGCTAGCGATGTGTCTCAGTGACG(+CAGTGT)GTACCCTTGCACATGGAGGAAGCCCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605110445_8w86 CTCGAATCCAAAACTGTATAGACGTTCCAAAG(+CAGTGT)GTACCTGTTTTCTAAGCTCTAATCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605110444_2ps8 GAAAGAGAAGAGGATTAGAGCTTAGAAAACAG(+CAGTGT)GTACCTTTGGAACGTCTATACAGTTTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605110441_5in7 ACAAGACAACACCACCACGGAAGCAGAGGGGG(+CAGTGT)GATCCATGCACAGCCTTTTCAGGGTCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605110440_s99s AGCCCCAAGAGACCCTGAAAAGGCTGTGCATG(+CAGTGT)GATCCCCCCTCTGCTTCCGTGGTGGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605110438_38r3 TCACATCTCCCTTCAGAAATCATGTTTGCAGG(+CAGTGT)GATCCGGTTTTGTATTTTGTTTTTAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240605110437_15dh TTAGACTTGTTTAAAAACAAAATACAAAACCG(+CAGTGT)GATCCCTGCAAACATGATTTCTGAAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605110435_lkxm GGACCTTTATTTACCTAACTTAAGATCATTAG(+CAGTGT)GTACCTGGTTTGGCTTACACTCAAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605110433_t9eh GCAGACCTGGGTTTGAGTGTAAGCCAAACCAG(+CAGTGT)GTACCTAATGATCTTAAGTTAGGTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605110432_d74q GTTGGGGGTGGGGTGTTACATGCATGTCACTG(+CAGTGT)GATCCTATGGAAGTTGTGAGCTGCCATGTAG NGG 10 8 17 8 24 0 100 5 10