Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110430_mdxj AGCAGCTACATGGCAGCTCACAACTTCCATAG(+CAGTGT)GATCCAGTGACATGCATGTAACACCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240605110428_5qf2 AGAGGGCCAATGAAACCCAAAGGTGAGAAAGG(+CAGTGT)GATCCTGGGCACAAGTGAGATAAACTTAATC NGG 10 8 17 8 24 0 100 5 10
    240605110426_hsgh CTTAGGATTAAGTTTATCTCACTTGTGCCCAG(+CAGTGT)GATCCCTTTCTCACCTTTGGGTTTCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605110425_n8ie GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+CAGTGT)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605110423_thxh TCTATTATTTTAGCTGGGGATACGTTTGGATG(+CAGTGT)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605110421_y9o6 AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605110419_1l9l ACAGATAGCTCCCCTGGATCTTATCAAAATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605110418_obf9 TAGTCCACTGCCAAGTGTGGCCCTAGGCATGG(+CAGTGT)GATCCCGGGGGCTCCAGGAAAATGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605110414_4qps ATGACACAATAGCATTTTCCTGGAGCCCCCGG(+CAGTGT)GATCCCATGCCTAGGGCCACACTTGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605110413_qwqm AAAAAAACAAACAAAGCAAAAACAAACAAAAG(+CAGTGT)GATCCCCTAGAAACAGTAAGGTAAATCTATT NGG 10 8 17 8 24 0 100 5 10