Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163356_7h5v TGAAAGAGCTTTTGTAACTCAAAGATTCAAAG(+AGGACA)GATCCGAGCTAATAGCCAGTTAGGTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240613163355_kznr CAAGGTCCACTCAACTATTCTGGCTTCTGTGG(+AGGACA)GTACCAGGCACACATGTGTAGTACATCCACA NGG 10 8 17 8 24 0 100 5 10
    240613163353_cpne GCATGTGTGGATGTACTACACATGTGTGCCTG(+AGGACA)GTACCCACAGAAGCCAGAATAGTTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613163351_l45y CTTTGTCGAAAGCTTTCATGTCAAAAGTAAAG(+AGGACA)GTACCCATCGTGACCAGGATTGCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240613163350_i6aq CAGGCATAGTTAAGCAATCCTGGTCACGATGG(+AGGACA)GTACCTTTACTTTTGACATGAAAGCTTTCGA NGG 10 8 17 8 24 0 100 5 10
    240613163348_e7bl GTTTTGGTCAAGCTGAGCTAGTCTGAGGTATG(+AGGACA)GTACCTCAGAATTACACAATAGCACCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613163347_qgnw AGAGGGAGAGGGTGCTATTGTGTAATTCTGAG(+AGGACA)GTACCATACCTCAGACTAGCTCAGCTTGACC NGG 10 8 17 8 24 0 100 5 10
    240613163346_esnu TGACTTCATTGAGTGTTCTCAGAAGAGCGCTG(+AGGACA)GTACCTTTTATAAAAGATTGTGGATCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613163344_8v06 TCAATCTGAAGATCCACAATCTTTTATAAAAG(+AGGACA)GTACCAGCGCTCTTCTGAGAACACTCAATGA NGG 10 8 17 8 24 0 100 5 10
    240613163343_tu58 CCTGTGTGATGTCAAATCCTCAGCCTGATCTG(+AGGACA)GTACCTTTCCTCTTTCCCACCTGCTGAGGCC NGG 10 8 17 8 24 0 100 5 10