Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163445_xqnm GTTTATCCAACTTCTGCCTTACTTTGCCACAG(+AGGACA)GTACCCACACACCCTGGACTTGAGCGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240613163443_93gd TTTGGTGCTCAGTAAAGGCCTGCAGAGCCTCG(+AGGACA)GATCCCGTTTGTGCTTTGAAGTCCTCACATG NGG 10 8 17 8 24 0 100 5 10
    240613163442_1lf2 AATTGCATGTGAGGACTTCAAAGCACAAACGG(+AGGACA)GATCCGAGGCTCTGCAGGCCTTTACTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613163440_8zm3 ATGTTCCTACCGCGGATGCTTTCAGTGCATAG(+AGGACA)GATCCTCAAGCAACATGAACCTGAAATAAGC NGG 10 8 17 8 24 0 100 5 10
    240613163439_1ja3 ACTGGGCTTATTTCAGGTTCATGTTGCTTGAG(+AGGACA)GATCCTATGCACTGAAAGCATCCGCGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613163437_akmw TCAATAATTTTTATCAGATTCTTCTCTGTAGG(+AGGACA)GTACCTTTCTCATATGTTCTGATTATTTACA NGG 10 8 17 8 24 0 100 5 10
    240613163436_7loq TACAGTGTAAATAATCAGAACATATGAGAAAG(+AGGACA)GTACCCTACAGAGAAGAATCTGATAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240613163434_ra4t GATCTGTATGTATACCACATAATGCGTGCCAG(+AGGACA)GTACCCACAGAGGTCATAAGAGGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613163432_9tkr AGGGGATCTTACCCCTCTTATGACCTCTGTGG(+AGGACA)GTACCTGGCACGCATTATGTGGTATACATAC NGG 10 8 17 8 24 0 100 5 10
    240613163431_7wc5 AGGTACACTGAGCCCCATGCTGGAGTGAGTGG(+AGGACA)GTACCTGGTCATGAGAGATGCCCAGCTCAGC NGG 10 8 17 8 24 0 100 5 10