Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163520_lbhd ACCATTTTAATTTTTTTGTTTGTTTGTTTCAG(+AGGACA)GTACCAAGTCAAGTGACAAAGAGCTGGCACA NGG 10 8 17 8 24 0 100 5 10
    240613163518_2g38 CACAGTATGCACACAGACAACTGTGAATTCAG(+AGGACA)GTACCCTTCCACAAGCCTTCCCTCTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240613163517_b29y TGGAAAGTACAAGAGGGAAGGCTTGTGGAAGG(+AGGACA)GTACCTGAATTCACAGTTGTCTGTGTGCATA NGG 10 8 17 8 24 0 100 5 10
    240613163515_130i TGCTATCCTGGGTAAGAACGCCCTGAGTACGG(+AGGACA)GTACCATGCTCTGTTTACTTTAGGATCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613163514_bxwv GGTGCTGGAGATCCTAAAGTAAACAGAGCATG(+AGGACA)GTACCCGTACTCAGGGCGTTCTTACCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613163512_3a4c TAAAAACCCTCATCAAACTACTTCATTTCCAG(+AGGACA)GATCCCCACATTTCCACTTTTGTGAAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613163510_b71f TTTTAACAGCTTCACAAAAGTGGAAATGTGGG(+AGGACA)GATCCTGGAAATGAAGTAGTTTGATGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613163508_8a3c AGATGACTCTCCATGAGAAGACTTGAAAACTG(+AGGACA)GTACCAGGAGTGGGCTGAAGATTCCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240613163506_b6e0 ATGACGTCATGGGAATCTTCAGCCCACTCCTG(+AGGACA)GTACCAGTTTTCAAGTCTTCTCATGGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613163505_y7bb AGTCCCTCAAACTATACTGATGATCAAATAAG(+AGGACA)GATCCGAACCACTACTAGAAATGCCCAACTA NGG 10 8 17 8 24 0 100 5 10