Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163739_i0z2 GATCTCGTTTACCTCCTGTTTAGTGGGCTTTG(+AGGACA)GATCCTCCCTGTTAGTTCTTCATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613163737_pacy TAAGTAAAACCACATGAAGAACTAACAGGGAG(+AGGACA)GATCCAAAGCCCACTAAACAGGAGGTAAACG NGG 10 8 17 8 24 0 100 5 10
    240613163735_n8xy GCTGCATCTCAGATTGTGGGGGGTGGGAGGGG(+AGGACA)GATCCTAACCTCAGGCACAATAGCATTCATA NGG 10 8 17 8 24 0 100 5 10
    240613163734_m07b CTGTTTATGAATGCTATTGTGCCTGAGGTTAG(+AGGACA)GATCCCCCTCCCACCCCCCACAATCTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613163732_einw ATGTGTGCCCCAAATACTCATGCTGTTCAAAG(+AGGACA)GATCCTCTTGTCTGCTTCGCATAGGGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613163731_bizd CCACACTCCACCCTATGCGAAGCAGACAAGAG(+AGGACA)GATCCTTTGAACAGCATGAGTATTTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613163730_hec7 TAATAAGTCCGTTAAAAGCAGAGCCAGGACTG(+AGGACA)GATCCCGAAAGAATAGGCTTTTGAAACAATC NGG 10 8 17 8 24 0 100 5 10
    240613163728_xzlc CATGTGATTGTTTCAAAAGCCTATTCTTTCGG(+AGGACA)GATCCAGTCCTGGCTCTGCTTTTAACGGACT NGG 10 8 17 8 24 0 100 5 10
    240613163727_eyri TCACATCATTTTCTTCCAGTTATTGGCTACAG(+AGGACA)GATCCAAGGGGAAGGTTTTCTGCTTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613163724_woq5 CTGTGAGCTGGAAGCAGAAAACCTTCCCCTTG(+AGGACA)GATCCTGTAGCCAATAACTGGAAGAAAATGA NGG 10 8 17 8 24 0 100 5 10