Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163534_5a7n CTATCTGGCCTGTGCAGAAGACAAGTGAAGTG(+AGGACA)GATCCTGAAGAAATGACAGTTCACTGATCAC NGG 10 8 17 8 24 0 100 5 10
    240613163532_bqu5 ATGTCATCCACTGCTTGGGGAATTTTCCTCAG(+AGGACA)GTACCTACACACCCTGATATTTGAAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240613163531_csv0 TGAGTTTTGGTTTCAAATATCAGGGTGTGTAG(+AGGACA)GTACCTGAGGAAAATTCCCCAAGCAGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240613163529_b6xf CTAGGTAAAATAATATGCAGTAGTATAGTGTG(+AGGACA)GATCCATTGCAATTACTGGAAAATTGGTTTA NGG 10 8 17 8 24 0 100 5 10
    240613163528_8klx TATTTTAAACCAATTTTCCAGTAATTGCAATG(+AGGACA)GATCCACACTATACTACTGCATATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240613163527_d3wh CAAGTTGCTATGCAAGTGGAAGGACTGTTTTG(+AGGACA)GATCCCCAGCACCCATGTAAAGTCAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240613163525_bbei ACATACTCATTTGACTTTACATGGGTGCTGGG(+AGGACA)GATCCAAAACAGTCCTTCCACTTGCATAGCA NGG 10 8 17 8 24 0 100 5 10
    240613163524_zais AACCAAAAACAAAAAAACAAAACAGAGTAGAG(+AGGACA)GTACCATTTCATGAAAGAAAAATGAGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240613163523_g944 ACACACCCTTCTCATTTTTCTTTCATGAAATG(+AGGACA)GTACCTCTACTCTGTTTTGTTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613163521_jd9h TACACTGTGCCAGCTCTTTGTCACTTGACTTG(+AGGACA)GTACCTGAAACAAACAAACAAAAAAATTAAA NGG 10 8 17 8 24 0 100 5 10