Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032419_wzl2 TGTCAATGTCATGTTTACTTCGCATATGAATG(+ACTCAC)GATCCATTACCTTTTCCCAATTACTACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605032418_ive2 TAAGTTGCAGTATGGATCCCAGGATGTTGAAG(+ACTCAC)GTACCAGAACTGTGGGTCATCGCCTGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605032416_df3d GGGCTTTCCTCAGGCGATGACCCACAGTTCTG(+ACTCAC)GTACCTTCAACATCCTGGGATCCATACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605032415_8a2u ACATGTTAAAGTGAAGTCTAAGTTGCAGTATG(+ACTCAC)GATCCCAGGATGTTGAAGGTACCAGAACTGT NGG 10 8 17 8 24 0 100 5 10
    240605032413_ir6j GACCCACAGTTCTGGTACCTTCAACATCCTGG(+ACTCAC)GATCCATACTGCAACTTAGACTTCACTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605032411_1rg4 CCAAATAAAAGAACAGGGGAAATTATGGGCAG(+ACTCAC)GATCCTAGGCAATAACTAAAGGTATAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605032409_3y0v TTAGACAACCTATACCTTTAGTTATTGCCTAG(+ACTCAC)GATCCTGCCCATAATTTCCCCTGTTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605032408_i216 CTGAAGAATGTCACCACCTAGGACATGCCTTG(+ACTCAC)GTACCTGAAGTTTTCATAAAGGTTTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240605032406_0mh3 TTCATTTATGGAAACCTTTATGAAAACTTCAG(+ACTCAC)GTACCAAGGCATGTCCTAGGTGGTGACATTC NGG 10 8 17 8 24 0 100 5 10
    240605032405_01xj GGTGTCAGCATTTGGAAGCTGATTATAGGATG(+ACTCAC)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10