Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032403_o7ur AGATGGACCATCTAGTGATTGCCATATGCAGG(+ACTCAC)GATCCATCCTATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605032402_ywpp TACTGTGATTTTCATAACCCGTTGCCTTTTTG(+ACTCAC)GTACCAGAGCTACGTGGTTTGAATCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605032401_6dfn CATGTAGCCAGGATTCAAACCACGTAGCTCTG(+ACTCAC)GTACCAAAAAGGCAACGGGTTATGAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605032358_ctwz GGAGTCAAGGACTATAGTCAGGAGACCCCCAG(+ACTCAC)GTACCTGGTTCAGAGCAGTCCGGGATGATAG NGG 10 8 17 8 24 0 100 5 10
    240605032356_uazr TCGTCCTATCATCCCGGACTGCTCTGAACCAG(+ACTCAC)GTACCTGGGGGTCTCCTGACTATAGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605032355_t0hg GTGTAATTGACCTGAAACATATACACGAAAAG(+ACTCAC)GTACCCATTTCTTCTAATGCCTTTAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605032353_m2bl TGGAATATTTTTAAAGGCATTAGAAGAAATGG(+ACTCAC)GTACCTTTTCGTGTATATGTTTCAGGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605032352_gxdu CATAAGGACCCTTCATGAGGACCCTGCATAAG(+ACTCAC)GTACCCAGGAGGATCATCAGGGCTAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605032350_8ck1 GGGCCACATTTTAGCCCTGATGATCCTCCTGG(+ACTCAC)GTACCTTATGCAGGGTCCTCATGAAGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605032349_wcw3 AAGCAAAAGTATGTCGCCTTTTTCTGAACATG(+ACTCAC)GTACCATAGGAATGCTGGTGGCTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10