Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032259_jxtp GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605032257_4wpk CTTTAAATGATGTGGAAATGGACAGACTCTTG(+ACTCAC)GATCCCATGGCTTAATTTTGAACAGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605032255_tr4j GCCTAGTTCCCCTGTTCAAAATTAAGCCATGG(+ACTCAC)GATCCAAGAGTCTGTCCATTTCCACATCATT NGG 10 8 17 8 24 0 100 5 10
    240605032254_mbtl GAGCCCATCCCTGGCACTGCCTGGGGGGCTAG(+ACTCAC)GATCCAGAGGGTGGATAGCCCAGAGGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605032252_n7ig CTATCCTAGGCCTCTGGGCTATCCACCCTCTG(+ACTCAC)GATCCTAGCCCCCCAGGCAGTGCCAGGGATG NGG 10 8 17 8 24 0 100 5 10
    240605032250_4lfl TTCTTAACACTGACCATATTCGGGGGTGGGGG(+ACTCAC)GATCCAAAAATTACAAACCATTTACTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605032249_tw8h ATTTTCAACTAGTAAATGGTTTGTAATTTTTG(+ACTCAC)GATCCCCCCACCCCCGAATATGGTCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605032247_afyn TTGTTGTTTTTTTTTTTTCTTACTAAAAGTGG(+ACTCAC)GATCCTTTTAGGCAGGATTTTATTCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605032246_oyjj CTTTGGCTACAGAATAAAATCCTGCCTAAAAG(+ACTCAC)GATCCCACTTTTAGTAAGAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605032245_t2hw TAACCTCAGCCCTGAGTTCAGGGAGACAGGTG(+ACTCAC)GATCCCATGGACTCACTGGCTGGATAAGCTA NGG 10 8 17 8 24 0 100 5 10