Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032158_m4l0 ATGTTGAGCCTTATTAAAATATCTTAATTATG(+ACTCAC)GTACCTCGATCTATAGGACCCAATTTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605032156_tmbj TATCTCCATGTACTCATGTGAATATAGCCATG(+ACTCAC)GATCCTATAGTGAGCTATATCCTTACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032155_yvyg AAGGTAAAAAGTAAGGATATAGCTCACTATAG(+ACTCAC)GATCCATGGCTATATTCACATGAGTACATGG NGG 10 8 17 8 24 0 100 5 10
    240605032153_pgmg GCTGATGTGCAGGGGGAAGTAGGATGGCAGGG(+ACTCAC)GTACCTAGAGCCCCCTTGATAAATTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605032152_yfp8 AGGACATCTGAAATTTATCAAGGGGGCTCTAG(+ACTCAC)GTACCCCTGCCATCCTACTTCCCCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605032149_yi94 CAAATTCTTTCCCAGGGTCATGTTGCACCCTG(+ACTCAC)GATCCATATGCTACTTTGTTGGTCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605032148_pqbq ATTTCTTGCTGAGACCAACAAAGTAGCATATG(+ACTCAC)GATCCAGGGTGCAACATGACCCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605032147_rt88 CTTTTTTTTTCTTCTTTTTGAAACAATGCTTG(+ACTCAC)GATCCTGTTCTTGGGCACATGGGGCCATTTG NGG 10 8 17 8 24 0 100 5 10
    240605032146_1xrz GGGTCCAAATGGCCCCATGTGCCCAAGAACAG(+ACTCAC)GATCCAAGCATTGTTTCAAAAAGAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605032144_n1rd TCTAATATAGGAAAATAGGAAATCCACCAGTG(+ACTCAC)GTACCATCTCTCAACATAAACATATGTACTA NGG 10 8 17 8 24 0 100 5 10