Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032143_yf4j TCATCTAGTACATATGTTTATGTTGAGAGATG(+ACTCAC)GTACCACTGGTGGATTTCCTATTTTCCTATA NGG 10 8 17 8 24 0 100 5 10
    240605032142_gr1b CCAAAACCACATGGGATTTTGACAACAAACTG(+ACTCAC)GATCCTTGCTACAATATTAATGTTCTATTCT NGG 10 8 17 8 24 0 100 5 10
    240605032140_7ld6 AAGAAAGAATAGAACATTAATATTGTAGCAAG(+ACTCAC)GATCCAGTTTGTTGTCAAAATCCCATGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605032139_s46g CCCCACTCCATTTGACTGTCTCTTCTTCTGTG(+ACTCAC)GATCCTGCAGCAGAGTTTTATTTCAGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605032138_002a ATGAGTAAGACTGAAATAAAACTCTGCTGCAG(+ACTCAC)GATCCACAGAAGAAGAGACAGTCAAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605032136_8z9s TTTCTGGCTCCTAAACGCCTTTGAGCTTGTTG(+ACTCAC)GTACCCAGAGAAATAAACTAAAATTGTATGA NGG 10 8 17 8 24 0 100 5 10
    240605032135_3y6h TTTTTTCATACAATTTTAGTTTATTTCTCTGG(+ACTCAC)GTACCAACAAGCTCAAAGGCGTTTAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605032133_x1cz AGGTTTTATTGTTCTTAAACAAGGTCAGCCAG(+ACTCAC)GTACCAGATTCTAAAAAGAACAATTGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605032132_5q7w AGGGGGGGGGCAATTGTTCTTTTTAGAATCTG(+ACTCAC)GTACCTGGCTGACCTTGTTTAAGAACAATAA NGG 10 8 17 8 24 0 100 5 10
    240605032129_kb6s GAGTAAGCTCCAGCCCCCAGCACCTTCCCTGG(+ACTCAC)GTACCAAGAGTCATCCGAGGGGAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10