Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032043_qeb5 AACTTATAATAAAAGAAAATATAAGAAGCGTG(+ACTCAC)GATCCTTAAATATGTGACAAATACACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605032042_69o2 GATGTTCATTATTCCTAAATAGCAGCTCAAAG(+ACTCAC)GATCCCTTTGCTACACAAGCGCAATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605032040_f6h8 CTTAGGTTTTCATTGCGCTTGTGTAGCAAAGG(+ACTCAC)GATCCTTTGAGCTGCTATTTAGGAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240605032039_s3h8 AATGTGTGTATTTCTTTATCTCTTACTCTATG(+ACTCAC)GTACCATGAGATCATTCAGCCGTCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605032038_cfz9 ACCTTTGACAAGGACGGCTGAATGATCTCATG(+ACTCAC)GTACCATAGAGTAAGAGATAAAGAAATACAC NGG 10 8 17 8 24 0 100 5 10
    240605032037_7dp7 CTTGCCTACATATGGAGCAGTGACTGCTACTG(+ACTCAC)GATCCCTAAGCAATAGATTCATTTGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605032035_b2th TGTAATGCCTCCAAATGAATCTATTGCTTAGG(+ACTCAC)GATCCAGTAGCAGTCACTGCTCCATATGTAG NGG 10 8 17 8 24 0 100 5 10
    240605032034_bjl5 TTGGATCTAGCACATAATAACAAGGCATAGTG(+ACTCAC)GTACCTGCCTGTTCTTGTGCTGAGACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605032032_czas CTGCATACACGTCTCAGCACAAGAACAGGCAG(+ACTCAC)GTACCACTATGCCTTGTTATTATGTGCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605032030_v6f8 CCTGTTCTTGTGCTGAGACGTGTATGCAGTGG(+ACTCAC)GATCCTGAGGGCTTTCCTGGACAGTCAATTT NGG 10 8 17 8 24 0 100 5 10