Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031943_cam3 ATTTATTTGATTTGATCATTTCTTCACATGTG(+ACTCAC)GTACCAAATTCAACAATGAAGCACAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605031942_wa9e ACAACATTTTAGGACTTAAACATTTGATTTCG(+ACTCAC)GTACCAGATGAATTGCTAACCTGTTACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605031941_5h10 AAATTATCAGTAACAGGTTAGCAATTCATCTG(+ACTCAC)GTACCGAAATCAAATGTTTAAGTCCTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605031940_pqfk GTCTCGGATTTGCTTGTTTGAGACCTTCTAAG(+ACTCAC)GTACCAGTGCACACTTAGTTGCTTATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605031938_986f TAAGAGTGAAATAAGCAACTAAGTGTGCACTG(+ACTCAC)GTACCTTAGAAGGTCTCAAACAAGCAAATCC NGG 10 8 17 8 24 0 100 5 10
    240605031937_syt8 ATTAATATTTAGAGTAGGAAGCAGCCTCTTAG(+ACTCAC)GTACCACAGTTAAAAACTAAGAGAACGAACA NGG 10 8 17 8 24 0 100 5 10
    240605031936_wa8o AATTTTGTTCGTTCTCTTAGTTTTTAACTGTG(+ACTCAC)GTACCTAAGAGGCTGCTTCCTACTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240605031934_j352 TGGGAAAGTGTGGGATCACCCCCTGGCCTTTG(+ACTCAC)GATCCTCCCCCATATATACTTTCTAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240605031932_t676 CCGCAAAGAGTTAGAAAGTATATATGGGGGAG(+ACTCAC)GATCCAAAGGCCAGGGGGTGATCCCACACTT NGG 10 8 17 8 24 0 100 5 10
    240605031931_zzxb ATTTAGTGTGTCAAAACGAACCGTACTCAAAG(+ACTCAC)GATCCACTAGTGTCCAATTCAAAAATGGCTG NGG 10 8 17 8 24 0 100 5 10