Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031842_b7vm TTTCTGTTCTCTCTCCCCTGCTCTCCCTACAG(+ACTCAC)GTACCTACACACACACACACACACACATCCC NGG 10 8 17 8 24 0 100 5 10
    240605031841_umys GGCACAGGCTAACAGAATGGATACTTAAATAG(+ACTCAC)GATCCATCATTTTGTTGCATACAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605031840_gnh4 GAAGTGTGTTTCTTGTATGCAACAAAATGATG(+ACTCAC)GATCCTATTTAAGTATCCATTCTGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605031838_29ak AGAAGATTATACCGTCTCCTCAGCACTTTGTG(+ACTCAC)GATCCTTCTCCAAAATTGACCATATACTCAA NGG 10 8 17 8 24 0 100 5 10
    240605031837_724x TATGATTGAGTATATGGTCAATTTTGGAGAAG(+ACTCAC)GATCCACAAAGTGCTGAGGAGACGGTATAAT NGG 10 8 17 8 24 0 100 5 10
    240605031835_gnr0 GGGTCTGTCCCTGAATCTGTTGCCTGCCTGTG(+ACTCAC)GATCCCATTCCCTTAACTTGTCTGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605031833_gtf7 TCCCACTGAGACCAGACAAGTTAAGGGAATGG(+ACTCAC)GATCCACAGGCAGGCAACAGATTCAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605031832_j4wr AAGCCAACTAGAAAACAACTGGCGTTTATCAG(+ACTCAC)GTACCTTAGATTGGTCACTTATACAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605031831_y7la ATAAAATTAGCTGTATAAGTGACCAATCTAAG(+ACTCAC)GTACCTGATAAACGCCAGTTGTTTTCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605031830_5xfo ACACAGAGCAACCCTGTCTCTCAATACTAAGG(+ACTCAC)GATCCCTAGAGTCAAGGGCTCTGGGTAAAAC NGG 10 8 17 8 24 0 100 5 10