Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031759_j3vb CTATTCGTGTGACCCAGTGACTCGAGCAAGTG(+ACTCAC)GATCCACTCTACAGATTACAGAGTTGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605031757_kghh TCTGAAGTAGTAAAGTCCTTTTTCCAGGTTAG(+ACTCAC)GTACCAGTTCAGGGTAAGTAACTGGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605031756_6yn4 GTAGGGTCTCTCCAGTTACTTACCCTGAACTG(+ACTCAC)GTACCTAACCTGGAAAAAGGACTTTACTACT NGG 10 8 17 8 24 0 100 5 10
    240605031754_zf8e GCTCACAAACATCTATAAATGTAGCTCCATAG(+ACTCAC)GATCCAATGCCCCCATCTGCATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240605031753_o4ij GGCATTTTGTCTGCATGCAGATGGGGGCATTG(+ACTCAC)GATCCTATGGAGCTACATTTATAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605031751_70b2 GAACACTTTTTGTTTTATTATTCTAGTCTGGG(+ACTCAC)GATCCAGATTCCATAGCCATGGGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605031750_whjf CAGGCCCAGCAGTCCCATGGCTATGGAATCTG(+ACTCAC)GATCCCCAGACTAGAATAATAAAACAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605031748_ss47 TACAGCATCTGCCATGCTCCCTTCCATAGCCG(+ACTCAC)GTACCTTAAGTGAGTTCAATAGTTCCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605031747_vu4x GCCTTTCAAAGGAACTATTGAACTCACTTAAG(+ACTCAC)GTACCGGCTATGGAAGGGAGCATGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240605031746_90bh CAAAAATGAAGAGAGAAAACAGGCCTATTGTG(+ACTCAC)GTACCTTCTCAACAAGGCTTTCAGTAAGAGT NGG 10 8 17 8 24 0 100 5 10