Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031859_t0ff TACTCCAGTAACAATTCTAATGCAATTGACTG(+ACTCAC)GATCCCACCAACTGGGAAGATGAAGGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605031857_su4n TCCCTCCCCACCGACATGACTCCCAAAGTGGG(+ACTCAC)GTACCAGACAGCATGGGGGTTAAGATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605031856_8moh TAGGAAACTAATCTTAACCCCCATGCTGTCTG(+ACTCAC)GTACCCCACTTTGGGAGTCATGTCGGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605031853_artg GCTGGTTCCAGCTGCTGTTCCATGGGCCCTTG(+ACTCAC)GTACCAAGGACTCGTATGTGTCCCCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605031851_xjfj GCCCTTCAAATGGGGACACATACGAGTCCTTG(+ACTCAC)GTACCAAGGGCCCATGGAACAGCAGCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605031849_ot11 GGGGTATACTCCAGTTATCACCCAACCTGGGG(+ACTCAC)GTACCCTCCAGTTATCACCCAACCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605031847_eflg CATTAGTCTGGTGGTTGGGTGATAACTGGAGG(+ACTCAC)GTACCCCCAGGTTGGGTGATAACTGGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605031846_93me CGCTAACCTCCCCTACTCACCTTGGCAATCTG(+ACTCAC)GATCCAATGCTCACTGCCTTCCCTTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605031845_jskj AACTTAGCCCCAAGGGAAGGCAGTGAGCATTG(+ACTCAC)GATCCAGATTGCCAAGGTGAGTAGGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605031843_ga4u TGAGAGGGATGTGTGTGTGTGTGTGTGTGTAG(+ACTCAC)GTACCTGTAGGGAGAGCAGGGGAGAGAGAAC NGG 10 8 17 8 24 0 100 5 10