Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031959_6lur GTGAAAAAATGTGGGAGTAGAAAGAAAAATGG(+ACTCAC)GATCCATGTGCTTCCAATTTAAAATTTATGG NGG 10 8 17 8 24 0 100 5 10
    240605031956_ewpg GGCGGGAAACATGGAGGCCCGGCGGTAAGGGG(+ACTCAC)GATCCCGGGCGGGCCAATCTACAAATACGCG NGG 10 8 17 8 24 0 100 5 10
    240605031954_2dpx GGGGACGCGTATTTGTAGATTGGCCCGCCCGG(+ACTCAC)GATCCCCCTTACCGCCGGGCCTCCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605031952_g091 CCAACAGGGAAAAGTCACCTGACTCCATTTAG(+ACTCAC)GTACCCCAAAATTTCCCAACCATGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605031951_8d9m GGTGTATAGAAACATGGTTGGGAAATTTTGGG(+ACTCAC)GTACCTAAATGGAGTCAGGTGACTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605031949_zuld GTGGCATGCTTAGGGAAACTCTGGAAAGCTCG(+ACTCAC)GTACCATGCAAAGTGCCTGGCCCAGAACTAA NGG 10 8 17 8 24 0 100 5 10
    240605031948_y333 GAAACTTAGTTCTGGGCCAGGCACTTTGCATG(+ACTCAC)GTACCGAGCTTTCCAGAGTTTCCCTAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605031947_94gv CCGGGATATACTCTCATTCTCTCTTCTTCAAG(+ACTCAC)GTACCTGCATGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605031946_5su0 ACACACACACACACACACACACACACATGCAG(+ACTCAC)GTACCTTGAAGAAGAGAGAATGAGAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240605031944_v01m AGCACTGAGGTTGTGCTTCATTGTTGAATTTG(+ACTCAC)GTACCACATGTGAAGAAATGATCAAATCAAA NGG 10 8 17 8 24 0 100 5 10