Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032517_gu42 AAAGATACTGAGGTTTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032516_ilpg TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAAAACCTCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605032514_pxp6 AAAGATACTGAGGTTTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032512_cvdi TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605032510_2nry ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032509_vni7 ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605032507_g6hd ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032505_f8g9 GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605032503_4vpl ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032502_1dxm GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10