Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031930_l5up GGGCACAGCCATTTTTGAATTGGACACTAGTG(+ACTCAC)GATCCTTTGAGTACGGTTCGTTTTGACACAC NGG 10 8 17 8 24 0 100 5 10
    240605031929_qhhf TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+ACTCAC)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605031927_3glh CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+ACTCAC)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605031926_m41h CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+ACTCAC)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605031925_cwmi TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+ACTCAC)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605031923_iuyq CACTTAGGTCAGTGTATCAGGCTTGGTGGCAG(+ACTCAC)GTACCTGTTCATGCTGAGGCATCGTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605031922_clla GTGAGGCTGGCACGATGCCTCAGCATGAACAG(+ACTCAC)GTACCTGCCACCAAGCCTGATACACTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605031921_13zn ATGGCCTTGACTCCTTTGTGGCTGAGCTGTTG(+ACTCAC)GTACCAGTGCAGTTCACGGGTGTTAGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605031919_a4ik TCCTCCAGTCCTAACACCCGTGAACTGCACTG(+ACTCAC)GTACCAACAGCTCAGCCACAAAGGAGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605031918_ltaf TCAAGGAAGTAGATAATCAACAGTGGGAACTG(+ACTCAC)GTACCCTCTGTCCCCAGGCCTGATTTAGAAG NGG 10 8 17 8 24 0 100 5 10