Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605032242_ncug TTGATTAGCTTATCCAGCCAGTGAGTCCATGG(+ACTCAC)GATCCACCTGTCTCCCTGAACTCAGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605032241_9c2q TGGTATGAGGCAAAGCCTCTTGAACACAAATG(+ACTCAC)GTACCCTCTTCATAATGGCTTTATTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605032240_b7b2 GGATCCAAAGGAATAAAGCCATTATGAAGAGG(+ACTCAC)GTACCATTTGTGTTCAAGAGGCTTTGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605032238_epst GGTACCCTCTTCATAATGGCTTTATTCCTTTG(+ACTCAC)GATCCTATTAAAGATTCCCTGCAGCTTGTAC NGG 10 8 17 8 24 0 100 5 10
    240605032237_nxcf CACTGGTACAAGCTGCAGGGAATCTTTAATAG(+ACTCAC)GATCCAAAGGAATAAAGCCATTATGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605032235_ix62 GCCTCACAACACTCCTGTGAGGTAGATATGTG(+ACTCAC)GATCCCCATTTCGAGATGGGTTAATAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605032233_1n94 TCTGTGCCTCTATTAACCCATCTCGAAATGGG(+ACTCAC)GATCCACATATCTACCTCACAGGAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605032231_rv9n ATCTCAGCCTTGGAATGCATGCACAGACAGGG(+ACTCAC)GATCCTGAGAGCAAGCTGGCTAGTGAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605032230_pbwh ACTGTGAGTCTCACTAGCCAGCTTGCTCTCAG(+ACTCAC)GATCCCCTGTCTGTGCATGCATTCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605032229_8nts GACTAACTATAAAATGAACAATCTACATATTG(+ACTCAC)GATCCATTCATTCCAAGATTAAAATTATCAC NGG 10 8 17 8 24 0 100 5 10