Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031245_t651 CAGCGTCCACTAGATTCCACAGTTAGCACTTG(+ACTCAC)GTACCAGGAGAAGAAAAGTCAATGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240605031243_hi1z GTTTTGGATTTGTCCCATTTAAAGAACTAATG(+ACTCAC)GATCCAGGGAAAATAAAGTATCCTTGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605031241_0c75 TGAGATCAAACAAGGATACTTTATTTTCCCTG(+ACTCAC)GATCCATTAGTTCTTTAAATGGGACAAATCC NGG 10 8 17 8 24 0 100 5 10
    240605031239_onu0 TGAAAGCATGTTAAGCACTAATACTAACCATG(+ACTCAC)GATCCTTATCCCTTAAGCAATAAAAAGATGT NGG 10 8 17 8 24 0 100 5 10
    240605031238_i22t AATGCACATCTTTTTATTGCTTAAGGGATAAG(+ACTCAC)GATCCATGGTTAGTATTAGTGCTTAACATGC NGG 10 8 17 8 24 0 100 5 10
    240605031237_qyas ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ACTCAC)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605031233_zcm7 TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ACTCAC)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605031232_8rgw ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ACTCAC)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605031229_kp0u TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ACTCAC)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605031228_tzqu ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ACTCAC)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10