Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031139_1t5p TATAATGTATGCCTTCTCCAGAGTCAGTGCAG(+ACTCAC)GATCCACCTGAGTGTGTATGTGTGAAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605031138_x7oh GTGGCCAACTTTATTTTTACGTGGAACATTAG(+ACTCAC)GTACCAAGTTTACAACCATCAAGCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605031136_6wsd GAACTATGTTTTGCTTGATGGTTGTAAACTTG(+ACTCAC)GTACCTAATGTTCCACGTAAAAATAAAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605031135_4jfw GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+ACTCAC)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605031134_6wjh ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+ACTCAC)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240605031133_5zro CCTTTTAGAAATAGGGTCTCCCTCTCTGTGTG(+ACTCAC)GTACCACTGGCTAAGGAAACCGTACTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605031131_r4vx AGGCCTCCCAAGTACGGTTTCCTTAGCCAGTG(+ACTCAC)GTACCACACAGAGAGGGAGACCCTATTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605031129_3krx GAGCCCGCCTGACACCCAAAGTTGTTAAGGAG(+ACTCAC)GATCCAAATTCCAGAGTTAAGACGCAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605031128_z37o GCGGGCACTCTGCGTCTTAACTCTGGAATTTG(+ACTCAC)GATCCTCCTTAACAACTTTGGGTGTCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240605031127_owij TAGAGCGCTCGCCTGGAGTACTTCAGGTTCTG(+ACTCAC)GATCCCCAGGCTCCAAAAAACCGAAACAAAA NGG 10 8 17 8 24 0 100 5 10