Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031624_e8zw ACCTTTAGGACAGATGCTTAGATGTGACATTG(+ACTCAC)GATCCTTGCCCTGGAGGAGGTGGGCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605031623_b929 GCTGGCCCATTTCATTTTGTCAGGGAGCTTAG(+ACTCAC)GTACCTGTGTCCTTGTTTTCCTAAAGCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605031622_37w7 GTGGGATCAGCTTTAGGAAAACAAGGACACAG(+ACTCAC)GTACCTAAGCTCCCTGACAAAATGAAATGGG NGG 10 8 17 8 24 0 100 5 10
    240605031621_d7w8 AGATGAGAGACATCAAGGCATAGTAATTTGAG(+ACTCAC)GATCCCATAAAGCATTTCTGAGGTGGCCATG NGG 10 8 17 8 24 0 100 5 10
    240605031619_7cde CCTGGCATGGCCACCTCAGAAATGCTTTATGG(+ACTCAC)GATCCTCAAATTACTATGCCTTGATGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605031618_piys AAAAGGCATGGGGCAGGGTACACTGGGGTTTG(+ACTCAC)GATCCACTTTCCTACCTCCTTCTGCTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605031616_l84a ATGTGAGCAAAGCAGAAGGAGGTAGGAAAGTG(+ACTCAC)GATCCAAACCCCAGTGTACCCTGCCCCATGC NGG 10 8 17 8 24 0 100 5 10
    240605031615_do1i TTGTTGTATTTGAACATTGTAAAGTGTGGGTG(+ACTCAC)GTACCTTAAACTGAATATGTGAAGAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605031614_1az7 AGTTTCTGGATTCTTCACATATTCAGTTTAAG(+ACTCAC)GTACCACCCACACTTTACAATGTTCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240605031612_5sno TGTGGGGTTTTAATTGGTCAGTTTTAATTTGG(+ACTCAC)GATCCCAAAGTTTTAACCTCCATTCAGGAAG NGG 10 8 17 8 24 0 100 5 10