Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031125_1t1i TTTGCTTTTGTTTCGGTTTTTTGGAGCCTGGG(+ACTCAC)GATCCAGAACCTGAAGTACTCCAGGCGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605031123_6uxv CTGTGGGATTCTGAGGACTTCCACAGCACTGG(+ACTCAC)GATCCAGCAGAAGGAAGGACAGTTTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605031122_ybig GACTCTTTTTCAAACTGTCCTTCCTTCTGCTG(+ACTCAC)GATCCCAGTGCTGTGGAAGTCCTCAGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605031121_mfr4 GAAAGAGCTTTGTCTTTAAAAAAGAGATCAAG(+ACTCAC)GATCCCAAAGAATACGATTCTCATATGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605031119_f5d5 ATTTTCTCACATATGAGAATCGTATTCTTTGG(+ACTCAC)GATCCTTGATCTCTTTTTTAAAGACAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605031118_n3q5 GACCTCAACTACTCCAAAAGCTGGGCTAGGAG(+ACTCAC)GATCCCAAGTTCTAGGCCAGCCAGGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605031116_vhxa CCCGAAGTTGCCCTGGCTGGCCTAGAACTTGG(+ACTCAC)GATCCTCCTAGCCCAGCTTTTGGAGTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605031114_c62q TCCCTCACCTGGCCAGCTGCCTGCCCAACAGG(+ACTCAC)GATCCTCCAGAGAGGGGGTAGCAATAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605031113_nvnq ATCCAGTTTCTATTGCTACCCCCTCTCTGGAG(+ACTCAC)GATCCCTGTTGGGCAGGCAGCTGGCCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605031112_85h8 TGTAACAGGATTACACAGGCTTCATTCGAGAG(+ACTCAC)GATCCTACACTAAGCCGTCTCTCCACACCCA NGG 10 8 17 8 24 0 100 5 10