Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031055_j11w AATGTCAACCATGCACATGGTCCTCATATGAG(+ACTCAC)GATCCTGTCTTAATGTTGTTGCCATTACTAC NGG 10 8 17 8 24 0 100 5 10
    240605031054_rgwv TGGTAAATAAGGCCTTCCTAGATGTGCACATG(+ACTCAC)GTACCAGGAGAATTGTTGCCCTGGGACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605031052_k57t TGGACCTTTGTCCCAGGGCAACAATTCTCCTG(+ACTCAC)GTACCATGTGCACATCTAGGAAGGCCTTATT NGG 10 8 17 8 24 0 100 5 10
    240605031051_tobt ACTCTCAAGGCATGACCTGAAATTGGAAATGG(+ACTCAC)GTACCATCTCTCCCCTCAGTCACTGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605031050_eu5n TTGAAGCTTTCCAGTGACTGAGGGGAGAGATG(+ACTCAC)GTACCCATTTCCAATTTCAGGTCATGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605031048_lq9s AAACACGGCTTTCCTAAGTAACTGTACCAATG(+ACTCAC)GATCCTAAAGTCCCAAATGTAGGCCCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605031047_epiv TTTGTTTCAAGGGCCTACATTTGGGACTTTAG(+ACTCAC)GATCCATTGGTACAGTTACTTAGGAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240605031045_wzt3 GCTTTTTTCCCCTTCTAAAGTTCAAGTAAAGG(+ACTCAC)GTACCTTTAAGAACAATCTGAATAGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605031044_7yh0 GCCTGCAGTCCCTATTCAGATTGTTCTTAAAG(+ACTCAC)GTACCCTTTACTTGAACTTTAGAAGGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605031042_c16x ACCACATGGTGGCTCAGTACCATCTGTAATGG(+ACTCAC)GATCCAATTCCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10