Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031009_w4u7 GGTTAAGAGAGGGGACCTTAACCGTTCCTTTG(+ACTCAC)GTACCCTGTTTGAGCAGGGTTGGTAATATCT NGG 10 8 17 8 24 0 100 5 10
    240605031008_r3g9 TGTTCCAAGACACACCCCTTCTAGAGATGCTG(+ACTCAC)GATCCAACCCAAACCTGCCCACTCCGGAACC NGG 10 8 17 8 24 0 100 5 10
    240605031006_5rj0 TGGTGGGTTCCGGAGTGGGCAGGTTTGGGTTG(+ACTCAC)GATCCAGCATCTCTAGAAGGGGTGTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605031005_n52j TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+ACTCAC)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605031003_cx27 GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+ACTCAC)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605031001_fcis TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+ACTCAC)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605030959_dtgo GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+ACTCAC)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605030958_fcpi ATTGCGGACCATGGATATATTTTATTAACTGG(+ACTCAC)GTACCCAAAACACATTTCCCATATTTGGATG NGG 10 8 17 8 24 0 100 5 10
    240605030956_t2p8 TTCACCATCCAAATATGGGAAATGTGTTTTGG(+ACTCAC)GTACCCAGTTAATAAAATATATCCATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605030954_r69s TGGTAGTGTTCAGCGTTGCCGTCAAGTAGAGG(+ACTCAC)GTACCTCAAAATATATGGTCCTTGTCCCTCG NGG 10 8 17 8 24 0 100 5 10