Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030938_dkws TTAGCCAGCAGGCAGTAGTGCTTGGTGTTAGG(+ACTCAC)GATCCCAGGGTTGCACCAAACCTTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605030936_dr2p CTCTAGAGAACCCTGACTAATAACAGAAGTTG(+ACTCAC)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605030935_rcol CTTGTACTTCTGTTACTCTAGAACCACTCCTG(+ACTCAC)GTACCAACTTCTGTTATTAGTCAGGGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605030933_rlk2 TCCTCAGAAAATTGGACATAGTACTACCAGAG(+ACTCAC)GATCCCGCAATGTCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605030931_fela TTCTGGATATATGCCCAGGAGAGACATTGCGG(+ACTCAC)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605030929_ekix GGATGGACCATCTAGAGACTGCCATATCTGGG(+ACTCAC)GATCCATCCCATAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605030928_vii3 GGTGTCAGCGTTTGGAGGCTGATTATGGGATG(+ACTCAC)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605030926_3w3o CTAAACAAACTTGGAGGTAGTCACTCAGGATG(+ACTCAC)GATCCCAATTCACACTTCTCCATAGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605030925_k5qf CAATCATGGAACTATGGAGAAGTGTGAATTGG(+ACTCAC)GATCCATCCTGAGTGACTACCTCCAAGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605030923_ufb7 GGAGGCCTAGAGAGCTCACAGTATTGAGTGAG(+ACTCAC)GTACCCCTTCCACTGGTTTCCATAGCACCCC NGG 10 8 17 8 24 0 100 5 10