Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031041_ihk5 GCTGTCTTCAGACACACTAGAAGAGGGAATTG(+ACTCAC)GATCCCATTACAGATGGTACTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605031040_bbte TGTATGTCAGTGATGGGGCTTGCCCGGGGAAG(+ACTCAC)GATCCAGAGATGAAGTAGAAGGAAAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605031038_ppjw GCAGGGAGTCTTTTCCTTCTACTTCATCTCTG(+ACTCAC)GATCCTTCCCCGGGCAAGCCCCATCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240605031037_7qix ACGTAGGCATGCAGTCAGTAGAAGGAGGGAGG(+ACTCAC)GTACCAACTGTGGTCAGGAGCTGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605031035_6tfa CTACTTCCCAGAACAGCTCCTGACCACAGTTG(+ACTCAC)GTACCCTCCCTCCTTCTACTGACTGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240605031033_wboh AGGGTCAGGGCTGTTGTGAGGACTAACTCCGG(+ACTCAC)GTACCCTTTTCCTGTTCCTTTCCCCCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605031031_hkqe GCCTGGAACAGGGGGAAAGGAACAGGAAAAGG(+ACTCAC)GTACCCGGAGTTAGTCCTCACAACAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605031029_bfah CCACATTTGGAAGGATGGAAGTCTAAGTGGGG(+ACTCAC)GTACCTGAAGGAGCTCTGTCTCTGTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605031028_qthb ATTGGGCAGAGACAGAGACAGAGCTCCTTCAG(+ACTCAC)GTACCCCCACTTAGACTTCCATCCTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605031026_4f2c AGTGCTTAAGAGTGCTTACTGTACTTCCAAAG(+ACTCAC)GATCCAAGTCTGCTCCCATGCAGGGCTCCCA NGG 10 8 17 8 24 0 100 5 10