Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031110_5kee AGAGATGGGTGTGGAGAGACGGCTTAGTGTAG(+ACTCAC)GATCCTCTCGAATGAAGCCTGTGTAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605031109_xf22 TGAGTCTGTAATGGACACACATTCCTGGATGG(+ACTCAC)GTACCCACAGCTCTACCATATCATATATTAA NGG 10 8 17 8 24 0 100 5 10
    240605031107_ucgo GTTGGTTAATATATGATATGGTAGAGCTGTGG(+ACTCAC)GTACCCATCCAGGAATGTGTGTCCATTACAG NGG 10 8 17 8 24 0 100 5 10
    240605031105_9aee CGATTTGATATCACAAGTCGAATGACTGTTGG(+ACTCAC)GATCCTGGAACCAATCATTCCTGAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605031104_ic1a TCATTCACAAACTCAGGAATGATTGGTTCCAG(+ACTCAC)GATCCCAACAGTCATTCGACTTGTGATATCA NGG 10 8 17 8 24 0 100 5 10
    240605031102_a4nq GGCTTGGATAGGTGGAATGGGAAAAGGTCCAG(+ACTCAC)GATCCTAAATAGCCCCTTCAAGGGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605031101_ba7g ACCAGGTCCATGCCCTTGAAGGGGCTATTTAG(+ACTCAC)GATCCTGGACCTTTTCCCATTCCACCTATCC NGG 10 8 17 8 24 0 100 5 10
    240605031059_xhxx TACAATCCATTGCTTCCATGAGAGATGGGCAG(+ACTCAC)GTACCTATCGGATGTGTGCTCTGCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605031058_dm94 GGAGAGAAACATGCAGAGCACACATCCGATAG(+ACTCAC)GTACCTGCCCATCTCTCATGGAAGCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605031057_po3a GTTTAGTAGTAATGGCAACAACATTAAGACAG(+ACTCAC)GATCCTCATATGAGGACCATGTGCATGGTTG NGG 10 8 17 8 24 0 100 5 10