Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030904_hfhn CCCAGGAACCCGTGAAGGCTGGCTGCTCTGGG(+ACTCAC)GATCCTCAAACTTCCTATTTCCACGTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605030903_as2o AGGCTACGTGCAGTATCTGCAATTCACGGATG(+ACTCAC)GATCCATCACTTTGGGTCTGAGGTACGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605030901_a9cd ATGTTTAGCCGTACCTCAGACCCAAAGTGATG(+ACTCAC)GATCCATCCGTGAATTGCAGATACTGCACGT NGG 10 8 17 8 24 0 100 5 10
    240605030900_rf28 GTACAATGGTGGCTTTAGCAACCACAAAGCTG(+ACTCAC)GATCCAGAAGTACTCATTAGTGTTGTATTTG NGG 10 8 17 8 24 0 100 5 10
    240605030859_fm7h AGATGCAAATACAACACTAATGAGTACTTCTG(+ACTCAC)GATCCAGCTTTGTGGTTGCTAAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605030858_v56x GAAGGAAGAGGATACGGATCACAAGGTCTCTG(+ACTCAC)GATCCATGGATTTCTGACTGCCTTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605030856_sbnf ACAGCTTTAGACAAGGCAGTCAGAAATCCATG(+ACTCAC)GATCCAGAGACCTTGTGATCCGTATCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605030854_y12v AAAAATCAAAGCACTCATTCACTTGGGGGGAG(+ACTCAC)GATCCTACGCAGGGGGGACTTGGTGCGTATA NGG 10 8 17 8 24 0 100 5 10
    240605030853_42rp CTTAATATACGCACCAAGTCCCCCCTGCGTAG(+ACTCAC)GATCCTCCCCCCAAGTGAATGAGTGCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605030851_lfzw GAGAAGGCCCTGTCTCCTCCTCAAGCCAAAGG(+ACTCAC)GATCCCAGCAGCAGCATCGTGGTGGCATCTT NGG 10 8 17 8 24 0 100 5 10