Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030807_pbrc AAGAAATGCACTAGAAATAGGAAAGTAACCAG(+ACTCAC)GTACCAAGAACTCTTGCAGAGCAAATCAATC NGG 10 8 17 8 24 0 100 5 10
    240605030806_akwj CTGCAGATTGATTTGCTCTGCAAGAGTTCTTG(+ACTCAC)GTACCTGGTTACTTTCCTATTTCTAGTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605030804_zbxw ACCACCACGCGGGTGGTGGGCATCGAACCTGG(+ACTCAC)GTACCCTGGAAGAGCAGCCAGTGCTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605030802_mtwp TAACAGTTAAGAGCACTGGCTGCTCTTCCAGG(+ACTCAC)GTACCCAGGTTCGATGCCCACCACCCGCGTG NGG 10 8 17 8 24 0 100 5 10
    240605030800_cwqy AGATGATCAAAGCACTGCAGGAATTAGGTGTG(+ACTCAC)GTACCCAGGGCCTGTAACCACAGTCAACTAG NGG 10 8 17 8 24 0 100 5 10
    240605030757_5t7h TCTAGCTAGTTGACTGTGGTTACAGGCCCTGG(+ACTCAC)GTACCACACCTAATTCCTGCAGTGCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605030756_qr2n TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+ACTCAC)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605030755_0uca CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+ACTCAC)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605030754_oda5 CACTATCACCGACATTCCTTCCAGCCACACTG(+ACTCAC)GATCCAGGAAACCTGTGCTGTTACAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605030752_frk1 GAACATTTAGCTGTAACAGCACAGGTTTCCTG(+ACTCAC)GATCCAGTGTGGCTGGAAGGAATGTCGGTGA NGG 10 8 17 8 24 0 100 5 10