Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030721_ggnj TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+ACTCAC)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605030719_pxz1 GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+ACTCAC)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605030717_ds7h CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+ACTCAC)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605030716_hqfs GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+ACTCAC)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605030714_n3bd TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+ACTCAC)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605030713_7vos CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+ACTCAC)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605030712_1d7e TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+ACTCAC)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605030710_v61s GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+ACTCAC)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605030708_ue6u CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+ACTCAC)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605030707_nq2z GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+ACTCAC)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10