Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030631_s46a GTGAGGTGGGAGAAGTGTGTATACCCCTCCTG(+ACTCAC)GATCCTGAATGTTTCTGCCTTTCCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605030629_hqjy CCCTCCCCCAAAGGAAAGGCAGAAACATTCAG(+ACTCAC)GATCCAGGAGGGGTATACACACTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605030628_1u9b ATCACAGACAATACAGCTTTTAACAAGCTGAG(+ACTCAC)GTACCTTTCTTTTTTTAGTGCTAATGATTCA NGG 10 8 17 8 24 0 100 5 10
    240605030627_23p8 TAGGTTGAATCATTAGCACTAAAAAAAGAAAG(+ACTCAC)GTACCTCAGCTTGTTAAAAGCTGTATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605030625_9l5n GTTAGCACCTAGAGGCCGAATCTAACGTCCCG(+ACTCAC)GATCCCATGCCTTGACACCCACAGCCCAATC NGG 10 8 17 8 24 0 100 5 10
    240605030623_3enm TCTAAGATTGGGCTGTGGGTGTCAAGGCATGG(+ACTCAC)GATCCGGGACGTTAGATTCGGCCTCTAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605030621_txde GAAGTCTGTGGAGCCAGTGAGGAAGACTCCTG(+ACTCAC)GATCCCTCCCCGTGATGGGTTAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605030619_nq30 CAGCTCTGTGGGGTTAACCCATCACGGGGAGG(+ACTCAC)GATCCAGGAGTCTTCCTCACTGGCTCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605030618_n2mt CAGAAAGTACCGCTTCCACTGAGACAGTGCTG(+ACTCAC)GATCCTGCCGAGCACTGCTGATCCCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605030617_m5ce TGCATCCCTCTGGGATCAGCAGTGCTCGGCAG(+ACTCAC)GATCCAGCACTGTCTCAGTGGAAGCGGTACT NGG 10 8 17 8 24 0 100 5 10